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c1000 touch thermal cycler  (Bio-Rad)


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    Structured Review

    Bio-Rad c1000 touch thermal cycler
    C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 22432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+real+time+system+c1000+thermal+cycler/CFX96+Touch+Deep+Well+Real-Time+PCR+Detection+System/pm42833467-85-24-28
    Average 96 stars, based on 22432 article reviews
    c1000 touch thermal cycler - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Software:

    Article Title: Epstein-Barr virus infection promotes T cell dysregulation in a humanized mouse model of multiple sclerosis.
    Article Snippet: .. Reactions were made up in Mx3000P 96- well plates (Agilent Technologies, no. 401333), and data were acquired using a CFX96 Real- Time System C1000 Thermal Cycler (Bio- Rad) and CFX manager 3.1 software for HEX detection. .. The cycler parameters were set according to the QIAGEN QuantiNova Probe PCR protocol for 2 min at 95°C to activate DNA polymerase and then 45 repeats of 5 s at 95°C and 5 s at 60°C.

    other:

    Article Title: Supporting Information Imidazo[2,1-b]benzothiazol derivatives as potential allosteric inhibitors of the glucocorticoid receptor
    Article Snippet: The RNA concentration and purity were evaluated by the Nano Drop instrument (NanoDrop 2000, EuroClone, Milan, Italy).

    Article Title: Epstein-Barr virus infection promotes T cell dysregulation in a humanized mouse model of multiple sclerosis
    Article Snippet: An EBV + B95-8 cell line extract (100 ng per reaction (American Type Culture Collection, no. CRL-1612) was used as a positive control for EBV detection.

    Article Title: A feed-forward loop between Toll/NF-κB and Rac1 promotes epithelial to mesenchymal transition of Ras-oncogenic hindgut enterocytes in Drosophila
    Article Snippet: For each qPCR reaction 6 μl cDNA, 4 μl gene specific primers and 10 μl KAPA SYBR Supermix (Kapa Biosystems) were used.

    Amplification:

    Article Title: Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor‐Dependent Manner
    Article Snippet: TaqMan assays were used for human TBX21 (Hs00894392_m1), RORC (Hs01076112_m1), GATA3 (Hs00231122_m1), BCL6 (Hs00153368_m1), and CHRNA7 (Hs01063372_m1), with PPIA (Hs99999904_m1) as the housekeeping control. .. Amplification was carried out on a CFX96 Real‐Time System C1000 Thermal Cycler (Bio‐Rad Laboratories) with the following cycling conditions: 50°C for 2 min, 95°C for 20 s, followed by 50 cycles of 95°C for 3 s and 60°C for 30 s. Gene expression levels were normalized to the reference gene PPIA , and the relative expression levels of TBX21 , RORC , GATA3 , and BCL6 were calculated using the 2 −ΔΔC t method. ..

    Article Title: Uncovering the association of lignin biosynthesis and accumulation with Huangguan plum fruit hollowness and browning by integrated physiological and proteomic analysis.
    Article Snippet: Plums are economically important stone fruits, valued for their nutritional quality and health-promoting properties.. However, their quality and marketability are often compromised by a physiological disorder called fruit hollowness and browning (HB).. This study employed an integrated physiological, biochemical, and proteomic approach to investigate the molecular mechanisms underlying HB in Huangguan plum.

    Gene Expression:

    Article Title: Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor‐Dependent Manner
    Article Snippet: TaqMan assays were used for human TBX21 (Hs00894392_m1), RORC (Hs01076112_m1), GATA3 (Hs00231122_m1), BCL6 (Hs00153368_m1), and CHRNA7 (Hs01063372_m1), with PPIA (Hs99999904_m1) as the housekeeping control. .. Amplification was carried out on a CFX96 Real‐Time System C1000 Thermal Cycler (Bio‐Rad Laboratories) with the following cycling conditions: 50°C for 2 min, 95°C for 20 s, followed by 50 cycles of 95°C for 3 s and 60°C for 30 s. Gene expression levels were normalized to the reference gene PPIA , and the relative expression levels of TBX21 , RORC , GATA3 , and BCL6 were calculated using the 2 −ΔΔC t method. ..

    Expressing:

    Article Title: Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor‐Dependent Manner
    Article Snippet: TaqMan assays were used for human TBX21 (Hs00894392_m1), RORC (Hs01076112_m1), GATA3 (Hs00231122_m1), BCL6 (Hs00153368_m1), and CHRNA7 (Hs01063372_m1), with PPIA (Hs99999904_m1) as the housekeeping control. .. Amplification was carried out on a CFX96 Real‐Time System C1000 Thermal Cycler (Bio‐Rad Laboratories) with the following cycling conditions: 50°C for 2 min, 95°C for 20 s, followed by 50 cycles of 95°C for 3 s and 60°C for 30 s. Gene expression levels were normalized to the reference gene PPIA , and the relative expression levels of TBX21 , RORC , GATA3 , and BCL6 were calculated using the 2 −ΔΔC t method. ..

    Reverse Transcription:

    Article Title: Docosahexaenoic acid supplementation inhibits monocyte exhaustion memory formation during sepsis
    Article Snippet: Samples were analyzed using a FACS Canto II (BD Biosciences). .. Total RNA was extracted using a RNeasy Plus Mini Kit (QIAGEN) and reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s protocol. qRT-PCR was performed using Power SYBR Green Master Mix (Thermo Fisher) on a CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad). ..

    Quantitative RT-PCR:

    Article Title: Docosahexaenoic acid supplementation inhibits monocyte exhaustion memory formation during sepsis
    Article Snippet: Samples were analyzed using a FACS Canto II (BD Biosciences). .. Total RNA was extracted using a RNeasy Plus Mini Kit (QIAGEN) and reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s protocol. qRT-PCR was performed using Power SYBR Green Master Mix (Thermo Fisher) on a CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Docosahexaenoic acid supplementation inhibits monocyte exhaustion memory formation during sepsis
    Article Snippet: Samples were analyzed using a FACS Canto II (BD Biosciences). .. Total RNA was extracted using a RNeasy Plus Mini Kit (QIAGEN) and reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s protocol. qRT-PCR was performed using Power SYBR Green Master Mix (Thermo Fisher) on a CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad). ..

    Isolation:

    Article Title: Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile
    Article Snippet: .. Once sample collection was complete, antennae were removed from RNALater and RNA was extracted using an SV Total RNA Isolation Kit, and cDNA was generated using a SensiFAST cDNA Synthesis Kit, with input RNA standardized based on input tissue. qPCR was conducted using a SensiFAST SYBR no-ROX Kit on a CFX96 Real Time System/C1000 Thermal Cycler (Bio-Rad Laboratories, Hercules, CA). (see Fig. S1 for protocol). ..

    Generated:

    Article Title: Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile
    Article Snippet: .. Once sample collection was complete, antennae were removed from RNALater and RNA was extracted using an SV Total RNA Isolation Kit, and cDNA was generated using a SensiFAST cDNA Synthesis Kit, with input RNA standardized based on input tissue. qPCR was conducted using a SensiFAST SYBR no-ROX Kit on a CFX96 Real Time System/C1000 Thermal Cycler (Bio-Rad Laboratories, Hercules, CA). (see Fig. S1 for protocol). ..

    cDNA Synthesis:

    Article Title: Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile
    Article Snippet: .. Once sample collection was complete, antennae were removed from RNALater and RNA was extracted using an SV Total RNA Isolation Kit, and cDNA was generated using a SensiFAST cDNA Synthesis Kit, with input RNA standardized based on input tissue. qPCR was conducted using a SensiFAST SYBR no-ROX Kit on a CFX96 Real Time System/C1000 Thermal Cycler (Bio-Rad Laboratories, Hercules, CA). (see Fig. S1 for protocol). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile
    Article Snippet: .. Once sample collection was complete, antennae were removed from RNALater and RNA was extracted using an SV Total RNA Isolation Kit, and cDNA was generated using a SensiFAST cDNA Synthesis Kit, with input RNA standardized based on input tissue. qPCR was conducted using a SensiFAST SYBR no-ROX Kit on a CFX96 Real Time System/C1000 Thermal Cycler (Bio-Rad Laboratories, Hercules, CA). (see Fig. S1 for protocol). ..



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